Troubleshooting Real-Time PCR Screening for Leishmania infantum
نویسندگان
چکیده
منابع مشابه
Quantification of Leishmania infantum DNA by a real-time PCR assay with high sensitivity.
A real-time PCR was developed to quantify Leishmania infantum kinetoplast DNA and optimized to reach a sensitivity of 0.0125 parasites/ml of blood. In order to analyze the incidence of heterogeneity and number of minicircles, we performed comparative PCR by using the Leishmania DNA polymerase gene as a reporter. Assays performed in both promastigote and amastigote stages showed variations among...
متن کاملParasite Burden in Hamsters Infected with Two Different Strains of Leishmania (Leishmania) infantum: “Leishman Donovan Units” versus Real-Time PCR
To develop and test new therapeutics and immune prophylaxis strategies for visceral leishmaniasis (VL), understanding tissue parasitism evolution after experimental infection with Leishmania infantum is important. Experimental infection in a hamster model (Mesocricetus auratus) reproduces several typical aspects of canine and human VL that are closely related to the inoculum's route. We quantif...
متن کاملComparison of real-time PCR and conventional PCR with two DNA targets for detection of Leishmania (Leishmania) infantum infection in human and dog blood samples.
Zoonotic visceral leishmaniasis (VL) is endemic in northwestern Iran. Real-time PCR, conventional PCR, and the direct agglutination test (DAT) were used to diagnose Leishmania infantum infection in blood samples from 100 domestic dogs and 100 humans. Based on clinical evaluation, 82 humans and 72 dogs from the endemic area were categorized as having asymptomatic infection, DAT positive with no ...
متن کاملPolymerase chain reaction and real-time PCR for diagnosing of Leishmania infantum chagasi in dogs Reação em Cadeia da Polimerase e PCR em tempo real para diagnóstico de Leishmania infantum chagasi em cães
The importance of dogs as a reservoir for Leishmania infantum chagasi in urban environments has stimulated numerous studies assessing diagnostic techniques. When performed properly, such procedures are an important step in preventing leishmaniasis in humans. Molecular methods have become prominent for this purpose. The aim of the present study was to determine the performance of the polymerase ...
متن کاملDiagnosis of infections with Leishmania infantum using PCR-ELISA.
On the basis of partial amplification of a cloned fragment of kDNA of Leishmania infantum which is specific for this species, we developed a PCR-ELISA technique which avoids the problems associated with classical diagnostic techniques. This technique was tested on 33 L. infantum strains from 19 different zymodemes, which were recognized equally. It was also used on human and canine clinical sam...
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ژورنال
عنوان ژورنال: Journal of Arthropod-Borne Diseases
سال: 2019
ISSN: 2322-2271,1735-7179
DOI: 10.18502/jad.v12i4.364